Project

Part:BBa_K2944003:Design

Designed by: Davindra Singh   Group: iGEM19_Concordia-Montreal   (2019-10-16)


pTDH3-GOx-tPGK1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 717
    Illegal BamHI site found at 1027
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1051
    Illegal BsaI.rc site found at 2269


Design Notes

Type IIs specific overhangs need to be accurate.


Source

The promoter and terminator come from the MoClo Toolkit on their respective plasmids, whereas the glucose oxidase gene sequence was obtained from a previous part, then optimized for yeast (BBa_K2238000).

References